Rapid urease test provides specific identification of Campylobacter pylori in antral mucosal biopsies.

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Rapid urease test provides specific identification of Campylobacter pylori in antral mucosal biopsies.

In a prospective study, three antral biopsies were taken from 175 dyspeptic patients during routine endoscopy. One biopsy was inserted immediately into a gel-containing well of a CLOtest slide and two biopsies were sent to histopathology. Using the CLOtest, 84 of the 175 samples (48%) detected urease activity in the gastric biopsy, suggesting infection with Campylobacter pylori. Histopathologic...

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Optimization of a medium for the rapid urease test for detection of Campylobacter pylori in gastric antral biopsies.

We developed a buffered azide-free urea medium which is sensitive, specific, and nontoxic for rapid detection of Campylobacter pylori in gastric biopsies. Detection of urease produced by the organism provides the basis for the test. The substrate is urea in monobasic sodium phosphate buffer, and phenol red provides indication of the pH change that results from urease activity. A rapid change fr...

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Investigation of H Pylori Colonization in Adenotonsillectomy Specimens by Means of Rapid Urease (CLO) Test

This prospective clinical study was performed to investigate the colonization of H pylori in adenoid and tonsil tissues by using the Campylobacter-like organism (CLO) test. Included in the study were 56 patients aged 3 to 43 yrs who had undergone adenoidectomy, tonsillectomy or adenotonsillectomy procedures under general anesthesia. Two-mm diameter tissue pieces of adenoid and tonsil specimens ...

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Evaluation of a rapid urease test to detect Campylobacter pylori infection.

Seventy-five consecutive patients referred for upper gastrointestinal tract endoscopy were evaluated for Campylobacter pylori infection by pathology, culture, and a biochemical test to detect bacterial urease. Forty-eight patients (64%) had C. pylori present based on pathology or culture or both. Thirty-two urease tests were positive after 1 h, all in patients with C. pylori detected by the two...

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Diagnosis of Helicobacter pylori infection using urease rapid test in patients with bleeding duodenal ulcer: influence of endoscopic signs and simultaneous corporal and antral biopsies.

INTRODUCTION The sensitivity of invasive diagnostic methods for Helicobacter pylori (H. pylori) infection, particularly of urease rapid test, is decreased in cases of gastroduodenal ulcer and upper gastrointestinal bleeding. OBJECTIVES To assess the influence of blood in the stomach or recent bleeding endoscopic signs in the diagnostic sensitivity of urease rapid test among patients with blee...

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ژورنال

عنوان ژورنال: Postgraduate Medical Journal

سال: 1988

ISSN: 0032-5473

DOI: 10.1136/pgmj.64.758.935